Purification of 45 KDa estramustine binding protein (EMBP) and preparation of its antibody.

نویسندگان

  • H Yuasa
  • H Yamanaka
  • K Imai
  • T Mashimo
  • H Kohno
چکیده

The purification of 45 KDa EMBP and the production of monospecific anti-serum is described. 45 KDa EMBP was purified by relatively simple methods using ion exchange HPLC (TSK-GEL DEAE-5PW column) and size exclusion HPLC (TSK-GEL G3000SW column). The results clearly demonstrated the speed and simplicity of the method using these columns, compared to previously-described methods for purification of 45 KDa EMBP.

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عنوان ژورنال:
  • Hinyokika kiyo. Acta urologica Japonica

دوره 36 2  شماره 

صفحات  -

تاریخ انتشار 1990